Global X now holds 8.68%, up from 7.46%.
This is the third time over the past month the fund has notified an increased change of interests.
Peninsula is producing uranium from the Lance Projects which have a mine life of at least 20 years, underpinned by 53.7 million pounds, the largest uranium ISR JORC-Code compliant resource in North America.
Peninsula has opened its in-the-money share purchase plan, offering shares at $0.50, which is the same price as the recent placement.
Following a rebound in the uranium price, Peninsula is trading at circa $0.74 a share.
Eligible shareholders can apply for either of the following denomination parcels: $2,500, $5,000, $7,500, $10,000, $12,500 or $15,000.
Peninsula added that applications may be scaled back based on demand, as the offer is seeking to raise $6.5 million.
Offer closes: 5.00 pm (Perth time) 3 March 2017.